| Date | Home | Score | Away | Venue |
| 10th May, 2016 | Altyn Asyr | - | Winner Play-off 1 | Ashgabat Stadium, Ashgabat |
| 10th May, 2016 | Al-Wehdat | - | Al-Ahed | King Abdullah II Stadium, Amman |
| 11th May, 2016 | Shabab Al-Dhahiriya | - | Winner Play-off 3 | Dora International Stadium, Hebron |
| 11th May, 2016 | Al-Jaish | - | Winner Play-off 4 | |
| 11th May, 2016 | Yangon United | - | Maziya | Thuwunna Stadium, Yangon |
| 11th May, 2016 | Lao Toyota FC | - | Ayeyawady United | New Laos National Stadium, Vientiane |
| 11th May, 2016 | Al-Muharraq | - | Fanja | |
| 11th May, 2016 | Mohun Bagan | - | South China | Howrah M.C.Stadium, Howrah |
| 11th May, 2016 | Johor Darul Ta'zim | - | Bengaluru FC | Larkin Stadium, Johor Bahru |
| 11th May, 2016 | Al-Oruba | - | Al-Quwa Al-Jawiya | Al-Seeb Stadium, Al-Seeb |